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H3757

Hyaluronidase aus Rindertestes

Type VIII, lyophilized powder, 300-1,000 U/mg

Synonym(e):

Hyaluronat-4-glycanhydrolase, Hyaluronoglucosaminidase

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Ihnen/SKUVerfügbarkeitPreis
100 mg
Warenkorb auf Verfügbarkeit prüfen
€ 171,00
10 x 100 mg
Warenkorb auf Verfügbarkeit prüfen
€ 585,00

Über diesen Artikel

CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
EG-Nummer:
Specific activity:
300-1,000 U/mg
Biological source:
bovine testis

€ 171,00


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biological source

bovine testis

Quality Segment

conjugate

conjugate (Glucosaminoglycan)

type

Type VIII

form

lyophilized powder

specific activity

300-1,000 U/mg

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

relevant disease(s)

cancer

storage temp.

−20°C

Gene Information

Biochem/physiol Actions

Diese Enzyme spalten randomisiert β-N-acetylhexosamin-[1→4]-glycosidische Bindungen in Hyaluronsäure, Chondroitin und Chondroitin-Sulfaten.
Hyaluronidase baut Hyaluronan ab und wird während der Krebsprogredienz unangemessen reguliert.

Preparation Note

Prepared from aseptic filled solution of Type I-S.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–6 of 6 Questions  
  1. My order of hyaluronidase (5 - 100mg bottles) was received at room temperature. The bottle states to store -20C. Is this product ok to use since it has been at room temp for several days?

    1 answer
    1. This item is stored frozen at -20°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time. Freezer storage products that ship at ambient temperature are considered stable up to 2 weeks for transit time.

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  2. The vials our lab got do not have expiration dates. What is the stability, unopened and opened, not reconstituted, when properly stored at -20C?

    1 answer
    1. This product is not assigned an expiration or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis.

      Helpful?

  3. What is the recommended method for reconstituting item H375, Hyaluronidase from bovine testes?

    1 answer
    1. The solubility of H375, Hyaluronidase from bovine testes, is 100 mg/mL in water. To achieve a higher solubility, it is advised to warm the tube at 37°C and shake it in an ultrasonic bath for a period. The stock solution can be stored at temperatures below -20°C for several months.

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  4. Good Morning, I our lab we got this product (H3757) and we would like to know how to obtain the package insert since we are not sure how to store it or after open expiration date. Not lot number on the bottle so not sure how to look for it. Thank you

    1 answer
    1. Hyaluronidase from bovine testes may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin. For more information please view the assay below:

      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase

      Helpful?

  5. How is product H3757, Hyaluronidase from bovine testes, solubilized?

    1 answer
    1. Product H3757, Hyaluronidase from bovine testes, may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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