G9768

Guanosin 5′-Triphosphat-Agarose

aqueous glycerol suspension

• Synonym(e): 5′-GTP agarose
• UNSPSC Code: 41106500
• NACRES: NA.56
• MDL number: MFCD00171655
• Biological source: bacterial (CORYNEBACTERIUM SP), enzyme from Porcine brain, plant (Sea weed), yeast
• Form: aqueous glycerol suspension
• Storage temp.: −20°C

Eigenschaften

• Quality Segment: 200
• biological source: bacterial (CORYNEBACTERIUM SP), enzyme from Porcine brain, plant (Sea weed), yeast
• form: aqueous glycerol suspension
• extent of labeling: 1-5 μmol per mL
• matrix: cross-linked 4% beaded agarose
• matrix activation: cyanogen bromide
• matrix attachment: ribose hydroxyls
• matrix spacer: 11 atoms (adipic acid dihydrazide)
• storage temp.: −20°C
• SMILES string: [X]O[C@H]1[C@@H](O[C@@H]([C@H]1O[X])CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O)[n]2c3nc(nc(c3nc2)O)N

Beschreibung

Application

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

Physical form

Suspension in 50% Glycerin, enthält 0.25 M NaCl und 0.02% Thiomersal

Guanosine 5′-triphosphate–Agarose has been used:

• in chromatographic column for the purification of a translocation stimulating factor
• in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)
• in GTP-Binding assay
• for the depletion of GTP binding proteins in cytosol of rat liver

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3