G5760

Glucose-6-phosphat-Dehydrogenase aus Leuconostoc mesenteroides

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

• Synonym(e): G-6-P-DH
• CAS-Nummer: 9001-40-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-602-6
• MDL number: MFCD00081656
• EG-Nummer: 1.1.1.49 (BRENDA, IUBMB)
• Specific activity: 550-1,100 units/mg protein (biuret)
• Biological source: bacterial (Leuconostoc mesenteroides)
• Concentration: ≥2.0 mg/mL Biuret

Eigenschaften

• biological source: bacterial (Leuconostoc mesenteroides)
• Quality Segment: 200
• type: Type XXIII
• form: ammonium sulfate suspension
• specific activity: 550-1,100 units/mg protein (biuret)
• mol wt: 54 kDa
• concentration: ≥2.0 mg/mL Biuret
• foreign activity: 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%, PGI ≤0.01%
• storage temp.: 2-8°C

Beschreibung

General description

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺). It corresponds to a molecular weight of 54 kDa and exists as a homodimer.

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:

• as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity
• as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes
• in the glucose-phosphorylating activity of chloroplast extracts
• as a model to test the effect of seed protein fractions on enzyme protection during dehydration.
• in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm. G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺) as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD⁺. G6PD uses NAD⁺ or NADP⁺ based on the catabolic or anabolic metabolic pathway.G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia.

Glucose-6-Phosphat-Dehydrogenase (G6PD) katalysiert die Umwandlung von Glucose-6-Phosphat zu 6-Phosphogluconolaceton und damit den ersten Schritt des Pentose-Phosphat-Wegs.

Physical form

Suspension in 2.7 M (NH₄)₂SO₄ solution containing 42 mM Tris and 0.8 mM MgCl₂

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)