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G5080

Sigma-Aldrich

Sephadex® G-50

Fine

Synonym(e):

Gel filtration resin, Sephadex G-50 Fine Medium

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About This Item

CAS-Nummer:
9048-71-9
UNSPSC-Code:
23151817
NACRES:
NA.56

Methode(n)

affinity chromatography: suitable

Aktive Matrixgruppe

phase

Quellen

1 g swells to 9-11 mL

Perlengröße

20-80 μm

Anwendung(en)

life science and biopharma

Kompatibilität

Cytiva

Verwandte Kategorien

Allgemeine Beschreibung

Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatography, and gel filtration chromatography. Sephadex is a beaded gel prepared by crosslinking dextran with epichlorohydrin.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000

Anwendung

Sephadex® G-50 has been used:
  • to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension
  • in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography
  • in desalting the recombinant enzymes eluted in the fraction five and six

Sephadex® G-50 is suitable for use in:

  • the separation of low and high molecular weight molecules
  • affinity chromatography, protein chromatography, and gel filtration chromatography

Leistungsmerkmale und Vorteile

  • Desalts, removes contaminants, and transfers to a new buffer in one step.
  • Suitable for DNA purification from small molecules using gel filtration.
  • Features a small bead size, resulting in shorter diffusion distances.
  • Considered a classic gel filtration resin.

  • Desalting with Sephadex is considered superior to dialysis because it saves time, has a low dilution factor, and recovers high activity even with minute amounts of sample

Sonstige Hinweise

G5080-100G′s updated product number is GE17-0042-01
G5080-500G′s updated product number is GE17-0042-02

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Journal of immunology (Baltimore, Md. : 1950), 151(5), 2877-2886 (1993-09-01)
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The New phytologist, 217(2), 896-908 (2017-10-11)
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