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F9765

Filipin complex

Name: Filipin complex
Brand: SIGMA
Price: 378 EUR

powder, ≥70% (UV)

Synonym(e):

Filimarisin

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Ihnen/SKU	Verfügbarkeit	Preis
25 mg	Warenkorb auf Verfügbarkeit prüfen	€ 378,00
50 mg	Warenkorb auf Verfügbarkeit prüfen	€ 696,00

Über diesen Artikel

CAS-Nummer:

11078-21-0

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00131073

€ 378,00

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Unterstützung erhalten

Eigenschaften

Produktname

Filipin-Komplex aus Streptomyces filipinensis, ≥70% (UV)

biological source

Streptomyces sp. (Streptomyces filipinensis)

Quality Segment

200

assay

≥70% (UV)

form

powder

solubility

methanol: 9.80-10.20 mg/mL, clear to hazy, yellow to very deep greenish-yellow

antibiotic activity spectrum

fungi

mode of action

cell membrane | interferes

storage temp.

−20°C

InChI

1S/C35H58O11/c1-4-5-11-16-31(42)34-33(44)22-29(40)20-27(38)18-25(36)17-26(37)19-28(39)21-32(43)23(2)14-12-9-7-6-8-10-13-15-30(41)24(3)46-35(34)45/h6-10,12-15,24-34,36-44H,4-5,11,16-22H2,1-3H3/b7-6+,10-8+,12-9+,15-13+,23-14+/t24-,25+,26-,27+,28-,29+,30+,31-,32+,33+,34-/m1/s1

InChI key

IMQSIXYSKPIGPD-YQRUMEKGSA-N

Beschreibung

General description

Chemische Struktur: Makrolid

Application

Filipin-Komplex aus Streptomyces filipinensis wird als profloureszierende Sonde zur Detektion von freiem Cholesterin in Zellen verwendet.

Biochem/physiol Actions

Filipin ist ein Polyen-Makrolid-Antibiotikum mit antimykotischer Wirkung. Der antimykotische Wirkmechanismus ist unklar, jedoch möglicherweise auf die Veränderung der Membrandurchlässigkeit und damit verbundenen Funktionen durch die Bindung an Membransterole zurückzuführen. Filipin bindet an Membransterole wie Cholesterin, hemmt die Endozytose des Prion-Proteins (PrP) und führt zur Freisetzung von PrP aus der Plasmamembran.

Filipin ist ein Polyen-Makrolid-Antibiotikum mit antimykotischer Wirkung. Der antimykotische Wirkmechanismus ist unklar, jedoch möglicherweise auf die Veränderung der Membrandurchlässigkeit zurückzuführen. Filipin bindet an Cholesterin enthaltende Lipid-Doppelschichtmodelle in kristalliner Phase sowie an Doppelschichten in flüssiger Phase mit oder ohne Cholesterin und richtet sich dabei so aus, dass sich Membranporen bilden.Es hemmt die Endozytose des Prion-Proteins (PrP) und führt zur Freisetzung von PrP aus der Plasmamembran.

Other Notes

Filipin enthält 8 isomere Komponenten, wobei Filipin III als Hauptkomponente gilt.

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1 of 1

Dieser Artikel	F4767	46745	O4876
Sigma-Aldrich F9765 Filipin-Komplex aus Streptomyces filipinensis	Sigma-Aldrich F4767 Filipin III aus Streptomyces filipinensis	Supelco 46745 Spiramycin aus Streptomyces sp.	Sigma-Aldrich O4876 Oligomycin aus Streptomyces diastatochromogenes
assay ≥70% (UV)	assay ≥85% (HPLC)	assay -	assay ≥90% total oligomycins basis (HPLC)
biological source Streptomyces sp. (Streptomyces filipinensis)	biological source -	biological source -	biological source -
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
form powder	form powder	form -	form powder
solubility methanol: 9.80-10.20 mg/mL, clear to hazy, yellow to very deep greenish-yellow	solubility methanol: 9.80-10.20 mg/mL, clear, colorless to yellow	solubility -	solubility -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–6 of 6 Questions

3 years ago

What is the stability of filipin when dissolved in DMSO and stored at -20 degrees C (how long can it be stored like this)?

1 answer
1. Technical Support
  
  10 months ago
  
  This product has not been tested for stability in solution, however product SAE0088 is a prepared 5/mg/mL solution in DMSO. This product is stable for at least 2 years at -20 deg. C. but does contain a proprietary stabilizer to enhance stability. See the link below to the product information sheet on the SAE0088 solution:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/973/858/sae0088pis.pdf
  Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate of Analysis for the prepared solution which includes the retest date:
  https://www.sigmaaldrich.com/product/sigma/f9765#product-documentation
  
  Helpful?
3 years ago

Which filipin is better to visualize cholesterol in hela cells by immunofluorescence - F9765 or F4767?

1 answer
1. Technical Support
  
  3 years ago
  
  Both F9765 and F4767 are suitable for determining cholesterol in cells. The F4767, Filipin III, is purified from Filipin. As it has a higher purity, it may offer a higher signal-to-noise ratio resulting in less background. These product have not been compared in side by side testing. It may be helpful to view the peer-reviewed knowledge articles to help with the appropriate selection:
  https://www.sigmaaldrich.com/search/f9765?focus=papers&page=1&perpage=30&sort=relevance&term=f9765&type=citation_search
  
  Helpful?
Anonymous

4 years ago

What is the Department of Transportation shipping information for this product?

1 answer
1. Technical Support
  
  4 years ago
  
  Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
  
  Helpful?
Anonymous

4 years ago

What is the difference between Filipin products F9765 and F4767?

1 answer
1. Technical Support
  
  4 years ago
  
  The product F9765 consists of a filipin complex. It is composed of 8 isomeric components, of which filipin III is the major component in the complex. F4767 consists of filipin III alone and is considered to be a better choice for staining.
  
  Helpful?
Anonymous

4 years ago

How should Product F9765, Filipin complex from Streptomyces filipinensis, be solubilized?

1 answer
1. Technical Support
  
  4 years ago
  
  This product has low solubility in aqueous buffers. It should initially be reconstituted in DMSO at 1 mg/mL and then diluted into saline or an aqueous buffer of choice.
  
  Helpful?
Anonymous

4 years ago

What is the stability of stock solutions Product F9765, Filipin complex from Streptomyces filipinensis?

1 answer
1. Technical Support
  
  4 years ago
  
  Solutions of filipin in aqueous or organic solvents are not considered to be stable and it is recommended that they be prepared fresh and not stored as stock solutions.
  
  Helpful?

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