F3046

Monoklonales Anti-Aktin

clone AC-40, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Monoclonal Anti-Actin
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: AC-40, monoclonal
• Application: IF (d)
• Citations: 7

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AC-40, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 42 kDa
• species reactivity: human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
• technique(s): direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTA1(58), ACTA1(58), ACTA1(58), ACTA2(59), ACTA2(59), ACTA2(59), ACTB(60), ACTC1(70), ACTC1(70), ACTC1(70), ACTG1(71), ACTG1(71), ACTG1(71), ACTG2(72), ACTG2(72), ACTG2(72) ; mouse ... Acta1(11459), Acta1(11459), Acta1(11459), Acta2(11475), Acta2(11475), Acta2(11475), Actb(11461), Actb(11461), Actb(11461), Actc1(11464), Actc1(11464), Actc1(11464), Actg1(11465), Actg1(11465), Actg1(11465), Actg2(11468), Actg2(11468), Actg2(11468) ; rat ... Acta1(29437), Acta1(29437), Acta1(29437), Acta2(81633), Acta2(81633), Acta2(81633), Actb(81822), Actb(81822), Actb(81822), Actc1(29275), Actc1(29275), Actc1(29275), Actg1(287876), Actg1(287876), Actg1(287876), Actg2(25365), Actg2(25365), Actg2(25365)

Beschreibung

General description

Der Antikörper erkennt ein Epitop am C-Terminus von Aktin, der in allen Aktin-Isoformen konserviert ist. Er markiert gezielt Aktin in einer Vielzahl von Geweben und Spezies. Das vom Antikörper erkannte Epitop ist resistent gegenüber Formalinfixierung und Paraffineinbettung. Zink-Formalin, B5, Ethanol, Methacarn und Brunnel- oder Bouin-Lösung können ebenfalls als Fixiermittel verwendet werden.

Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Immunogen

Synthetisches C-terminales Aktin-Peptid, das sich am Rückgrat von multiplen Antigenpeptiden (MAP) befindet

Application

Als primärer Antikörper für die Western-Blot-Analyse von MDCK-Zelllysaten wurde ein monoklonaler Anti-Aktin-Antikörper verwendet.

Monoklonaler Anti-Aktin-Antikörper wurde für die Western-Blot-Analyse von Cos-7-Zelllysaten verwendet, um eine gleichmäßige Beladung von Proteinen zu garantieren.

Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

Biochem/physiol Actions

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable