E8008

Ethylendiamintetraessigsäure -Lösung

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

• Synonym(e): EDTA -Lösung
• Empirische Formel (Hill-System): C₁₀H₁₆N₂O₈
• CAS-Nummer: 60-00-4
• Molekulargewicht: 292.24
• NACRES: NA.75
• PubChem Substance ID: 24894664
• eCl@ss: 39030907
• UNSPSC Code: 12352204
• MDL number: MFCD00003541
• Beilstein/REAXYS Number: 1716295

Eigenschaften

• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• concentration: 0.02% in DPBS (0.5 mM)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: 7.1-7.7
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
• InChI key: KCXVZYZYPLLWCC-UHFFFAOYSA-N

Beschreibung

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable