The Duolink Probemaker PLUS and MINUS have been optimized to conjugate the oligo arms of the PLA probes to antibodies, not proteins. Conjugation will happen in the amine groups. We do not have experience with conjugation of proteins or oligos with Probemaker. Therefore, we do not recommend it, nor can we provide technical support.
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DUO96040
Duolink® PLA Multicolor Probemaker Kit - Far Red
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
Synonym(e):
in situ Proximity Ligation Assay, Flowcytometry-PLA, Protein Protein Interaction Kit
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 996,00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
41105331
Fluorescence:
λex 646 nm; λem 664 nm (Far Red), λex 646 nm; λem 664 nm
Technique(s):
immunofluorescence: suitable, multiplexing: suitable, proximity ligation assay: suitable
Technischer Dienst
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Unterstützung erhaltenproduct line
Duolink®
technique(s)
immunofluorescence: suitable, multiplexing: suitable, proximity ligation assay: suitable
fluorescence
λex 646 nm; λem 664 nm (Far Red), λex 646 nm; λem 664 nm
suitability
suitable for fluorescence
shipped in
dry ice
storage temp.
−20°C
Application
Specificity
Far Red fluorescence detection reagents are often used with Cy®5 filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Far Red fluorescence detection reagents are often used with Cy®5 filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Features and Benefits
Multicolor Duolink® proximity ligation assay (PLA®) is an application of the Duolink® technologythat allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink® PLA Probemaker Guide for Multicolor Detection and the Duolink® PLA Multicolor Detection protocol to use this product.
Components
Duolink® PLA Multicolor Probemaker Kit - Far Red contains all the necessary reagents to perform the conjugations of Far Red Oligo H and Far Red Oligo I directly to primary antibodies. The resulting PLA probes (Far Red H and Far Red I) must be used together and cannot be paired with any other Duolink® PLA probe.
This product is comprised of the following:
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
- Detect multiple PPI, PTM and low abundant protein events in a single sample.
- Visualize protein complex formation and dissociation
- Monitor protein signaling pathways
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Detection of low abundant proteins due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Faster and more simple than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Components
Duolink® PLA Multicolor Probemaker Kit - Far Red contains all the necessary reagents to perform the conjugations of Far Red Oligo H and Far Red Oligo I directly to primary antibodies. The resulting PLA probes (Far Red H and Far Red I) must be used together and cannot be paired with any other Duolink® PLA probe.
This product is comprised of the following:
- Far Red Oligo H DUO86040H
- Far Red Oligo I DUO86040I
- Conjugation Buffer DUO82033
- Stop Reagent DUO82034
- Storage Solution DUO82035
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
Legal Information
Cy is a registered trademark of Cytiva
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
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Dieser Artikel | |||
|---|---|---|---|
| description Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events | description Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events | description Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events | description Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events |
| fluorescence λex 646 nm; λem 664 nm (Far Red) | fluorescence λex 594 nm; λem 624 nm (Texas Red®), λex 594 nm; λem 624 nm (red) | fluorescence λex 490 nm; λem 520 nm (FITC), λex 490 nm; λem 520 nm (green) | fluorescence λex 542 nm; λem 562 nm (orange) |
| technique(s) immunofluorescence: suitable, proximity ligation assay: suitable, multiplexing: suitable | technique(s) immunofluorescence: suitable, multiplexing: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, multiplexing: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, multiplexing: suitable, proximity ligation assay: suitable |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
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Is it possible to conjugate proteins with oligos for the PLA assay using the Probemaker?
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