As per our Enzymatic Assay of XYLANASE Activity in DRISELASE (EC 3.2.1.8):
Driselase (Xylanase) Enzyme Solution
(Immediately before use, prepare a solution containing 0.075 – 0.15 units/ml of Xylanase in
cold deionized water.) The pH information can also be found in the link below:
https://www.sigmaaldrich.cn/deepweb/assets/sigmaaldrich/product/documents/124/166/d9515enz.pdf
D8037
Driselase™ Basidiomycetes sp.
BioReagent, suitable for plant cell culture
Synonym(e):
Driselase™ aus Basidiomycetes ssp.
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1 G
€ 199,00
5 G
€ 650,00
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Unterstützung erhaltenproduct line
BioReagent
Quality Level
form
powder
composition
Protein, ≥10% biuret
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
−20°C
Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| form powder | form powder | form powder | form powder |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| product line BioReagent | product line BioReagent | product line BioReagent | product line BioReagent |
Application
Driselase™ Basidiomycetes sp. wird wie folgt verwendet:
- bei der Sphäroplastenherstellung aus Coccomyxa-Zellen
- in einem Mutageneseprotokoll auf Basis von CRISPR/Cas9 für Brachypodium distachyon und sein verwandtes Allopolyploid Brachypodium hybridum
- für den Zellwandaufschluss zum Durchführen der Ganzpräparatimmunlokalisierung von Lotus japonicus Wurzelgewebe
Biochem/physiol Actions
Driselase™ ist eine natürliche Mischung von Enzymaktivitäten (Kohlenhydrate aus Pilzen), die zum Aufschluss von Pflanzenzellwänden verwendet wird, um die Mazeration von Pflanzenmaterial, die Protoplastenbildung und Extraktionsprozesse zu erleichtern. Driselase setzt Zellwandkohlenhydrate frei. Diese Formulierung enthält die Enzymaktivitäten von Cellulose, Endo-1,3-β-glucanase und Xylanase.
Other Notes
Rohes Pulver mit Laminarinase, Xylanase und Cellulase.
Legal Information
Driselase is a trademark of ASKA Animal Health Co. Ltd.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
T Ishii et al.
Carbohydrate research, 248, 179-190 (1993-10-04)
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose
D P Blowers et al.
Plant physiology, 86(2), 505-509 (1988-02-01)
Plasma membrane vesicles from wild carrot cells grown in suspension culture were isolated by aqueous two-phase partitioning, and ATP-dependent phosphorylation was measured with [gamma-(32)P]ATP in the presence and absence of calcium. Treatment of the carrot cells with the cell wall
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8037-1G | 04061833589120 |
| D8037-5G | 04061833589137 |
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Hi, do you know dissolvability and the optimum pH of this enzyme?
1 answer-
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