D7191

Dibenzylfluorescein

• Synonym(s): DBF
• Empirical Formula (Hill Notation): C₃₄H₂₄O₅
• Molecular Weight: 512.55
• UNSPSC Code: 12171500
• PubChem Substance ID: 329798844
• MDL number: MFCD13184323

Properties

• assay: 99-102% (NMR)
• Quality Segment: 200
• form: solid
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: O=C1C=CC2=C(c3ccc(OCc4ccccc4)cc3OC2=C1)c5ccccc5C(=O)OCc6ccccc6
• InChI: 1S/C34H24O5/c35-25-15-17-29-31(19-25)39-32-20-26(37-21-23-9-3-1-4-10-23)16-18-30(32)33(29)27-13-7-8-14-28(27)34(36)38-22-24-11-5-2-6-12-24/h1-20H,21-22H2
• InChI key: YZJGKSLPSGPFEV-UHFFFAOYSA-N

Description

Application

DBF is dealkylated by cytochromes P450 (especially CYP2C8, CYP2C9, CYP2C19, and CYP3A) to form a fluorescein ester. Dibenzylfluorescein was reported to be suitable for in vitro assessment of the initial screening of CYP3A4-
mediated drug interactions. The fluorescent fluorescein product can be quantitated after the addition of base, which hydrolyzes the ester and increases the pH for optimal fluorescein fluorescence detection.

DBF is dealkylated by cytochromes P450 (especially CYP2C8, CYP2C9, CYP2C19, and CYP3A) to form a fluorescein ester. The fluorescent fluorescein product can be quantitated after the addition of base, which hydrolyzes the ester and increases the pH for optimal fluorescein fluorescence detection.

Other Notes

Dibenzylfluorescein is light sensitive and should be protected from direct sunlight or UV sources.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 + H312 + H332
• pcodes: P261 - P264 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• ppe: dust mask type N95 (US), Eyeshields, Gloves