The concentration of the DNA marker is not part of the specification and that information is not available. Product DPLUS-1K - DirectLoad™ Plus 1kb DNA Ladder is a comparable marker that may be considered an alternative. DPLUS-1KT is noted as having a DNA concentration of 100 ug/ul. The DNA concentration for individual bands is unavailable.
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D7058
DirectLoad™ Wide Range DNA Marker
ready-to-use marker for DNA electrophoresis
Synonym(e):
agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder
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Über diesen Artikel
NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenQuality Segment
form
liquid
usage
100 uses
solubility
water: soluble
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.
Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
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Dieser Artikel | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility water: soluble | solubility - | solubility water: soluble | solubility - |
| usage 100 uses | usage 100 uses | usage 75 uses | usage sufficient for 50 uses |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Could you provide the concentraion of the marker? Idealy band by band so that it can be used as an estimate of the concentration of samples run along side.
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