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D5523

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium, niedriger Glucosegehalt

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

DME, DMEM

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About This Item

MDL-Nummer:
MFCD00217342
UNSPSC-Code:
12352207
NACRES:
NA.75

Qualitätsniveau

500

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Anwendung

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).

Menge

Formulated to contain 10.0 grams of powder per liter of medium.

Rekonstituierung

Supplement with 3.7 g/L sodium bicarbonate.

Ergänzung

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317,H319

Gefahreneinstufungen

Eye Irrit. 2 - Skin Sens. 1

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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