D2691

Monoclonal Anti-DC-SIGN1/DC-SIGN2 antibody produced in mouse

clone 120612, purified immunoglobulin, lyophilized powder

• Synonym(e): Anti-CD209/CD299, Anti-DC-SIGN Related, Anti-DC-SIGN/DC-SIGNR, Anti-Dendritic Cell-Specific ICAM-3 Grabbing Non-Integrin
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03701938
• Conjugate: unconjugated
• Clone: 120612, monoclonal
• Application: FACS, IHC
• Citations: 10

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 120612, monoclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): flow cytometry: suitable, immunohistochemistry: 8-25 μg/mL using cell or in fixed tissue sections
• isotype: IgG2a
• UniProt accession no.: Q9H2X3
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CD209(30835), CLEC4M(10332)

Beschreibung

General description

DC-SIGN1 is a type II protein C-type lectin that binds mannose-rich molecules such as ICAM-3 and ICAM-2 in a calcium dependent manner. DC-SIGN1, a 44kDa molecule, is identical to the HIV-1 binding protein. Analysis of various tissues and cell lines suggests that DC-SIGN1 expression is restricted to dendritic cells, although there is evidence of expression in placenta, resting monocytes, and monocytic cell lines. DC-SIGN2 (DC-SIGNR), located on human chromosome 19p13.3, shares 73-80% amino acid identity with DC-SIGN1. DC-SIGN2 is polymorphic and is primarily transcribed in the liver and lymph nodes but not in monocyte-derived DC. Expression of DC-SIGN2 is restricted to endothelial cells derived from liver sinusoids, lymph nodes sinuses, and capillaries. Membrane and soluble forms of the protein have also been found to be expressed in placenta and some monocytic cell lines.

Immunogen

purified from NIH/3T3 cells transfected with recombinant human DC-SIGN2 (DC-SIGNR).

Application

Monoclonal Anti-DC-SIGN1/DC-SIGN2 antibody produced in mouse is suitable for flow cytometry and immunohistochemistry at a working concentration of 8-25ug/mL using cell or in fixed tissue sections.

Biochem/physiol Actions

DC-SIGN1 is involved in molecular interactions between dendritic cells and resting T-cells. DC-SIGN interacts with viruses such as X4, R5, X4R5 HIV-1 strains, HIV-2, and SIV. It captures HIV-1 through its interaction with the gp120 envelope glycoprotein and promotes efficient infection of CD4⁺ T cells that co-express chemokine receptors. DC-SIGN2 also binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120. It binds to human and simian immunodeficiency viruses and activates infection in trans.

Recognizes both human DC-SIGN1 and human DC-SIGN2 on transfected cells and reacts with human dendritic cells. The antibody does not react with parental mouse cells or irrelevant transfectants.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.