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D2625

5-Dimethylamino-1-naphthalinsulfonylchlorid

BioReagent, suitable for amino acid labeling, powder and chunks, ≥99% (HPLC)

Dansyl chloride

Synonym(e):

DNSCl, Dansylchlorid

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1 G

€ 77,90

5 G

€ 270,00

25 G

€ 1.040,00

€ 77,90


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Über diesen Artikel

Empirische Formel (Hill-System):
C12H12ClNO2S
CAS-Nummer:
Molekulargewicht:
269.75
UNSPSC Code:
12352101
NACRES:
NA.26
PubChem Substance ID:
EC Number:
210-092-6
Beilstein/REAXYS Number:
2217205
MDL number:

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InChI key

XPDXVDYUQZHFPV-UHFFFAOYSA-N

InChI

1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3

SMILES string

CN(C)c1cccc2c(cccc12)S(Cl)(=O)=O

product line

BioReagent

assay

≥99% (HPLC)

form

powder and chunks

color

yellow to yellow-orange

mp

72-74 °C (lit.)

solubility

acetone: 20 mg/mL

suitability

suitable for amino acid labeling

storage temp.

2-8°C

Quality Level

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Dieser Artikel
39220311155362891
assay

≥99% (HPLC)

assay

≥99.0% (HPLC)

assay

≥99% (HPLC)

assay

97%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

-

product line

-

form

powder and chunks

form

-

form

solid

form

liquid

solubility

acetone: 20 mg/mL

solubility

acetone: 0.2 g/10 mL

solubility

DMF: soluble, chloroform: soluble

solubility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Ein fluorogenes Reagens für die N-terminale Derivatisierung von Aminosäuren und Peptiden und die Detektion durch Umkehrphasen-HPLC.
Dansyl chloride has been used:
  • as a fluorogenic reagent for fluorescent N-terminal derivatization of amino acids and peptides and detection by reverse phase high performance liquid chromatography (HPLC)[1]
  • in the derivatization of plasma samples[2][3]
  • in the quantification of immobilized alkaline phosphatase[4]

Biochem/physiol Actions

Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins.[5] Dansyl chloride is used for derivatization of free amino acids and amines[6] in neural samples and biological fluids.[7] It is used to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization.[7]

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Lagerklasse

8A - Combustible corrosive hazardous materials

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

The use of dansyl-chloride for the detection of amino acids and serotonin in nervous tissue
Research Methods in Neurochemistry, 443-462 (1975)
Measuring the poise of thiol/disulfide couples in vivo
Jones DP and Liang Y
Free radical biology & medicine, 47(10), 1329-1338 (2009)
Simultaneous separation and quantitation of amino acids and polyamines of forest tree tissues and cell cultures within a single high-performance liquid chromatography run using dansyl derivatization
Minocha R and Long S
Journal of Chromatography A, 1035(1), 63-73 (2004)
Sarah A Goretta et al.
Bioorganic & medicinal chemistry, 20(13), 4012-4019 (2012-06-08)
Sphingomyelin (SM) and cholesterol form microdomains called lipid rafts in cellular membranes. To develop a versatile fluorescent lipid probe, chemical modifications to both the hydrophobic and hydrophilic portions of SM are essential. Few reports describing SM probes with a fluorophore
Shaodong Jia et al.
Journal of chromatography. A, 1218(51), 9174-9182 (2011-11-15)
A novel liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOFMS) method was developed for the simultaneous determination of 23 amino acids and 7 biogenic amines in food samples. These analytes were pre-column derivatized with dansyl chloride and then separated

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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