D2321

DAF-FM DA solution

5 mM in DMSO, ≥97% (HPLC)

• Synonym(e): 4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate
• Empirische Formel (Hill-System): C₂₅H₁₈F₂N₂O₇
• CAS-Nummer: 254109-22-3
• Molekulargewicht: 496.42
• MDL number: MFCD16872050
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥97% (HPLC)
• Form: DMSO solution
• Storage condition: desiccated, protect from light

Eigenschaften

• Quality Segment: 100
• assay: ≥97% (HPLC)
• form: DMSO solution
• storage condition: desiccated, protect from light
• color: clear colorless
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• InChI key: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Beschreibung

Biochem/physiol Actions

DAF-FM DA is a cell permeable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Other Notes

Light sensitive

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable