D225

4,5-Diaminofluorescein diacetate solution

Name: 4,5-Diaminofluorescein diacetate solution
Brand: SIGMA
Price: 769 EUR

5 mM in DMSO, ≥97% (HPLC)

Synonym(e):

DAF-2 DA

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1 MG

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Über diesen Artikel

UNSPSC Code:

41116100

NACRES:

NA.28

MDL number:

MFCD01866183

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Unterstützung erhalten

assay

≥97% (HPLC)

form

solution

availability

not available in Japan

concentration

5 mM in DMSO

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

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Dieser Artikel	D2813	D224	251505-M
Sigma-Aldrich D225 4,5-Diaminofluorescein diacetate solution	Sigma-Aldrich D2813 4,5-Diaminofluorescein diacetate	Sigma-Aldrich D224 4,5-Diaminofluorescein	Sigma-Aldrich 251505-M DAF-2 DA
concentration 5 mM in DMSO	concentration -	concentration 5 mM in DMSO	concentration -
form solution	form lyophilized powder	form solution	form solution
assay ≥97% (HPLC)	assay ≥98% (HPLC)	assay ≥97% (HPLC)	assay ≥98% (HPLC)
shipped in wet ice	shipped in wet ice	shipped in -	shipped in -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C

General description

4,5-Diaminofluorescein diacetate (DAF-2 DA) solution is a membrane-permeable fluorescent probe, which measures nitric oxide (NO) in living cells. It is used to study the synthesis of NO in bovine aortic endothelial cells (BAECs).[1] DAF-2 DA imaging shows that ciliated epithelial cells produces high amount of NO than non-epithelial inflammatory cells.[2]

Application

Cell-permeable fluorescent detector of nitric oxide in living cells.

Legal Information

DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590

Lagerklasse

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

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Determinants of shear stress-stimulated endothelial nitric oxide production assessed in real-time by 4, 5-diaminofluorescein fluorescence

Qiu W, et al.

Biochemical and Biophysical Research Communications, 286(2), 328-335 (2001)

Increased nitric oxide production in nasal epithelial cells from allergic patients-RT-PCR analysis and direct imaging by a fluorescence indicator: DAF-2 DA

Takeno S, et al.

Clinical and Experimental Allergy, 31(6), 881-888 (2001)

Endothelial Glycocalyx-Mediated Nitric Oxide Production in Response to Selective AFM Pulling.

Anne Marie W Bartosch et al.

Biophysical journal, 113(1), 101-108 (2017-07-13)

Nitric oxide (NO) is a regulatory molecule in the vascular system and its inhibition due to endothelial injury contributes to cardiovascular disease. The glycocalyx is a thin layer of glycolipids, glycoproteins, and proteoglycans on the surface of mammalian epithelial cells.

Site-specific phosphorylation and caspase cleavage of GFAP are new markers of Alexander disease severity.

Rachel A Battaglia et al.

eLife, 8 (2019-11-05)

Alexander disease (AxD) is a fatal neurodegenerative disorder caused by mutations in glial fibrillary acidic protein (GFAP), which supports the structural integrity of astrocytes. Over 70 GFAP missense mutations cause AxD, but the mechanism linking different mutations to disease-relevant phenotypes

Nitric oxide-coupled signaling in odor elicited molecular events in the olfactory center of the terrestrial snail, Helix pomatia.

Zoltán Serfőző et al.

Cellular signalling, 30, 67-81 (2016-11-26)

Olfaction, a chemosensory modality, plays a pivotal role in the orientation and behavior of invertebrates. The central olfactory processing unit in terrestrial stylomatophoran snails is the procerebrum, which contains NO synthesizing interneurons, whose oscillatory currents are believed to be the

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Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

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4,5-Diaminofluorescein diacetate solution