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D1821

Sigma-Aldrich

DAF-FM

≥98% (HPLC)

Synonym(e):

4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM

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About This Item

Empirische Formel (Hill-System):
C21H14F2N2O5
CAS-Nummer:
254109-20-1
Molekulargewicht:
412.34
UNSPSC-Code:
12352204
PubChem Substanz-ID:
329798711
NACRES:
NA.77

Qualitätsniveau

100

Assay

≥98% (HPLC)

Form

powder

Lagerbedingungen

desiccated

Farbe

white to brown

Löslichkeit

DMSO: >1 mg/mL

Versandbedingung

wet ice

Lagertemp.

−20°C

SMILES String

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChIKey

DIJCILWNOLHJCG-UHFFFAOYSA-N

Anwendung

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Biochem./physiol. Wirkung

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Vorsicht

hygroscopic

Angaben zur Herstellung

Color variation within the range from white to brown does not change performance.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Phillip Roder et al.
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Environmental health perspectives, 118(5), 589-595 (2010-05-05)
Silica nanoparticles (SiNPs) are being formulated for cellular imaging and for nonviral gene delivery in the central nervous system (CNS), but it is unclear what potential effects SiNPs can elicit once they enter the CNS. As the resident macrophages of
Visualization of Nitric Oxide, Measurement of Nitrosothiols Content, Activity of NOS and NR in Wheat Seedlings
Adavi SB, et al.
Bio-protocol, 9(20) (2019)
Roberto Villalobos-Labra et al.
Placenta, 86, 35-44 (2019-07-28)
Pregestational maternal obesity (PGMO) associates with foetoplacental vascular endothelial dysfunction and higher risk for insulin resistance in the neonate. We characterised the PGMO consequences on the insulin response of the human foetoplacental vasculature. Umbilical veins were from pregnancies where the
Hongwei Gao et al.
Cell death & disease, 8(8), e3004-e3004 (2017-08-18)
Tanshinones belong to a group of lipophilic constituents of Salvia miltiorrhiza Bunge (Danshen), which is widely used in traditional Chinese medicine. A deluge of studies demonstrated that tanshinones exert anti-inflammatory effects, but the underlying mechanisms remain unclear to date. This
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