The B9059 BACE-1 product included in the kit, serves as a positive control. The Technical Bulletin notes that the concentration of this item is approximate 3 units/microliter. This material has not been tested for purity. Therefore, a molar concentration cannot be provided.
CS0010
β-Secretase (BACE1) Activity Detection Kit (Fluorescent)
1 kit sufficient for 250 reactions
Synonym(e):
BACE1 Activity Detection Kit (Fluorescent)
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Über diesen Artikel
NACRES:
NA.84
UNSPSC Code:
12161503
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Unterstützung erhaltenusage
kit sufficient for 250 reactions
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... BACE1(23621)
Verwandte Kategorien
1 of 4
Dieser Artikel | S4195 | RAB0623 | AB5832 |
|---|---|---|---|
| Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) |
| usage kit sufficient for 250 reactions | usage - | usage - | usage - |
| Quality Level 300 | Quality Level 200 | Quality Level - | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Application
The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1.
Biochem/physiol Actions
BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery.
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Fluorescent Assay Buffer 50 mL
- Stop Solution 15 mL
- Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL
- Assay Standard 140 μL
- BACE1 (β−Secretase) 300 units 100 μL
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
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Piyoosh Sharma et al.
European journal of medicinal chemistry, 167, 510-524 (2019-02-21)
The multitarget-directed strategy offers an effective and promising paradigm to treat the complex neurodegenerative disorder, such as Alzheimer's disease (AD). Herein, a series of N-benzylpiperidine analogs (17-31 and 32-46) were designed and synthesized as multi-functional inhibitors of acetylcholinesterase (AChE) and
Maricarmen Hernández-Rodríguez et al.
Molecular neurobiology, 57(9), 3979-3988 (2020-07-09)
The increase of amyloid beta (Aβ) release and hyperphosphorylation of Tau protein represents the main events related to Alzheimer's disease (AD). Furthermore, the sporadic type represents the most common form of AD. Therefore, the establishment of a non-transgenic animal model
Lucas J Gutiérrez et al.
Journal of biomolecular structure & dynamics, 37(1), 229-246 (2018-01-06)
We report in this work new substituted aminopyrimidine derivatives acting as inhibitors of the catalytic site of BACE1. These compounds were obtained from a molecular modeling study. The theoretical and experimental study reported here was carried out in several steps:
Fluoro-benzimidazole derivatives to cure Alzheimer's disease: In-silico studies, synthesis, structure-activity relationship and in vivo evaluation for β secretase enzyme inhibition.
Sayyad Ali et al.
Bioorganic chemistry, 88, 102936-102936 (2019-05-06)
β-secretases as a target for the treatment of Alzheimer's.
Citron, M.
Journal of Neuroscience Research, 70, 373-379 (2003)
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what is the concentration of BACE1 enzyme in micromolar?
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