CRISPR16H

Lenti CRISPR HPRT1 gRNA Control Transduction Particles, High Titer (LV02 vector)

• NACRES: NA.51
• UNSPSC Code: 41106609
• Form: liquid
• Concentration: (5x10⁸ TU/ml (via p24 titering assay))

Eigenschaften

• form: liquid
• Quality Segment: 200
• packaging: vial of 8x25 μL (aliquots)
• concentration: (5x10⁸ TU/ml (via p24 titering assay))
• application(s): CRISPR
• shipped in: dry ice
• storage temp.: −70°C

Beschreibung

General description

The Lenti CRISPR HPRT1 gRNA Control Transduction Particles (LV02 vector) product is a critical positive control used to monitor transduction efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control uses a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and Ef1a-driven Puromycin resistance casette. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Application

Functional Genomics/Screening/Target Validation

Other Notes

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Legal Information

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable