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| Size/SKU | Availability | Price |
|---|---|---|
1 ea | Usually ships in 3 weeks. | $281.00 |
About This Item
NACRES:
NE.02
UNSPSC Code:
41106609
packaging
vial of 50 μL
Quality Segment
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−20°C
Application
Functional Genomics/Screening/Target Validation
To see more application data, protocols, vector maps visit Sigma.com/crispr.
- Positive control, HPRT1, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.
To see more application data, protocols, vector maps visit Sigma.com/crispr.
Features and Benefits
Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection.
Physical form
The Sigma CRISPR Lenti Human HPRT1 Positive Control Vector is provided as 1 μg of plasmid DNA in Tris−EDTA (TE).
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −70°C | storage temp. −70°C | storage temp. −20°C |
| concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) | concentration 5x108 VP/ml (via p24 assay) | concentration 1x106 VP/ml (via p24 assay) | concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| packaging vial of 50 μL | packaging vial of 200 μL | packaging vial of 200 μL | packaging vial of 50 μL |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| application(s) CRISPR | application(s) CRISPR | application(s) CRISPR | application(s) CRISPR |
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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