CLL1223

Safe Harbor Landing Pad Cell Line THP-1 Monocytes

human male peripheral blood (Source Disease: Acute monocytic leukemia)

• UNSPSC Code: 41106514
• NACRES: NB.01
• Biological source: human male peripheral blood (Source Disease: Acute monocytic leukemia)
• Growth mode: Suspension

Eigenschaften

• Produktname: Safe Harbor Landing Pad Cell Line THP-1 Monocytes,
• biological source: human male peripheral blood (Source Disease: Acute monocytic leukemia)
• Quality Segment: 200
• form: frozen liquid (Vial of Frozen Cells)
• growth mode: Suspension
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

The STR profile of this cell line matches that of its parental cell line ATCC^®Catalog No. TIB-202. THP-1 are a human monocyte cell line isolated from the peripheral blood of a male infant with acute monocytic leukemia. The cells are phagocytic and lack surface and cytoplasmic immunoglobulins. Differentiation can be induced with TPA.

Application

This product is a human THP-1 monocyte cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr^® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Biochem/physiol Actions

These cells were derived from the peripheral blood of a 1-year old male with acute monocytic leukemia. These cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. They stain positive for α-napthhyl butyrate esterase, produce lysozymes, and are phagocytic (both latex beads and sensitized erythrocytes). They can restore the response of purified T lymphocytes to Concanavalin A, show increased CO2 production on phagocytosis, and can be differentiated into macrophage-like cells using, for example, DMSO.

Features and Benefits

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These THP-1 cells are grown in suspension with a doubling time of approximately 24 hours.

Analysis Note

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

ATCC is a registered trademark of American Type Culture Collection

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Lagerklasse: 10 - Combustible liquids