DMS 79

95062824, human lung, Irregular

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: DMS 79, 95062824
• Biologische Quelle: human lung
• Wachstumsmodus: Aggregates in suspension
• Karyotyp: Aneuploid
• Morphologie: Irregular
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human lung small cell carcinoma

Beschreibung der Zelllinie

DMS79 has been established from pleural fluid of a patient with small cell carcinoma of the lung. The patient was treated with cytoxan, vincristine, methotrexate and radiation therapy. The cultures show a tendency for the modal chromosome number to decline. The production of adrenocorticotropin (ACTH), bombesin, calcitonin, β-endorphin, 17-β-estradiol, lipotropin, ocytocin-neurophysin, parathyroid hormone and somatstatin-like imuno-reactivity has been reported. The cells have also been reported to have the following cell surface antigens: Leu-7, Class I HLA, Class II HLA, Mg23 defined by the AML-2-23 monoclonal antibody and the AML-1-99 defined antigen. The cells have a receptor for epidermal growth factor (EGF) and produce tumours in nude athymic mice. Cells grow as irregularly shaped aggregates.

Anwendung

Human lung tumour model, carcinogenesis studies.

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subkultur-Routine

Maintain cultures between 3 ? 9 x 100,000 cells/ml; 5% CO₂; 37°C. Only dilute 1:2 as a maximum. This cell line grows as clumps in suspension which can be easily dispersed with pipetting. Trypsinisation is not necessary. Routine splitting can therefore

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