SK LU 1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93120835, human lung, Epithelial

• UNSPSC Code: 41106514
• Biological source: human lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 46
• Morphology: Epithelial

Eigenschaften

• biological source: human lung
• packaging: tube of 5 μg 93120835-DNA-5UG, pkg of vial of cells 93120835-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from a 64 year old Caucasian female with lung adenocarcinoma (grade III). The line is tumourigenic in immunotolerant rats. HLA = Aw24, w32, w41, B27

Human Caucasian lung adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].