COLO 677

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human blood
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: Not specified
• Morphology: Round & Refractile

Eigenschaften

• biological source: human blood
• packaging: tube of 5 μg 93072109-DNA-5UG, pkg of vial of cells 93072109-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Round & Refractile
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Tumourigenicity studies

Biochem/physiol Actions

COLO 677 was originally described as being derived from a tumour in the left axillary lymph node of a 39-year-old male with small lung cell carcinoma in 1989. However, DNA fingerprinting suggests cross-contamination with cell line RPMI-8226; RPMI-8226 was established from the peripheral blood of a 61-year-old male with multiple myeloma in 1966 (see DSMZ website)

Human multiple myeloma

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Will form clusters in suspension.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.