C0155

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human blood
• Technique(s): cell culture | mammalian: suitable
• Ethnicity: UK Caucasian
• Growth mode: Suspension
• Morphology: Lymphoblastoid

Eigenschaften

• biological source: human blood
• description: gel volume in water M
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 93021844-DNA-5UG, pkg of vial of cells 93021844-1VL
• HRC Panel Number: 3
• age at venesection: 58
• ethnicity: UK Caucasian
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: -140 to -196°C

Beschreibung

Application

As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

Biochem/physiol Actions

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes. This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.

UK Caucasian Male

Preparation Note

Maintain cultures between 3 x 10⁵ - 2 x 10⁶ cells/ml; 5% CO₂, 37 °C.

RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS). Once a growing culture is established the FBS concentration can be reduced to 10%.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.