Y13-259

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 89111412, no DNA, Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus

• UNSPSC Code: 41106514
• Biological source: Rat x Rat Hybridoma
• Isotype: IgG1
• Technique(s): cell culture | mammalian: suitable
• Myeloma: Y3Ag1.2.3
• Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus

Eigenschaften

• biological source: Rat x Rat Hybridoma
• packaging: pkg of vial of cells 89111412-1VL, tube of 5 μg 89111412-DNA-5UG
• manufacturer/tradename: ECACC
• ImmunoDon: Osborne-Mendel rat spleen
• Myeloma: Y3Ag1.2.3
• Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Immunogen

Ha-MuSV transformed syngeneic NRK cells

Biochem/physiol Actions

Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen

The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.

Preparation Note

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.