I-10

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: mouse testis
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 40, hyperdiploid
• Morphology: Epithelial

Eigenschaften

• biological source: mouse testis
• packaging: tube of 5 μg 89031605-DNA-5UG, pkg of vial of cells 89031605-1VL
• growth mode: Adherent
• karyotype: 2n = 40, hyperdiploid
• morphology: Epithelial
• products: Steroids
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).

Mouse BALB/c Leydig cell testicular tumour

Preparation Note

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].