HeLa (AC-free)

8011102, human cervix, Spherical

• UNSPSC-Code: 41106514

Eigenschaften

• product name: HeLa (AC-free), 08011102
• Biologische Quelle: human cervix
• Wachstumsmodus: Aggregates in suspension
• Karyotyp: 2n = 46, aneuploid
• Morphologie: Spherical
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human cervical carcinoma, serum-free, animal component (AC) free

Beschreibung der Zelllinie

HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).

Anwendung

Virus studies

Nährmedium

EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Subkultur-Routine

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Sonstige Hinweise

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