AFRC MAC 61.6

no DNA, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: Rat x Rat Hybridoma
• Isotype: IgG2c
• Technique(s): cell culture | mammalian: suitable
• Myeloma: YB2/3.0 Ag20
• Reactivity: Anti-Abscisic acid, plant growth hormone

Eigenschaften

• biological source: Rat x Rat Hybridoma
• packaging: tube of 5 μg 01010804-DNA-5UG, pkg of vial of cells 01010804-1VL
• ImmunoDon: F344 Rat spleen
• Myeloma: YB2/3.0 Ag20
• Reactivity: Anti-Abscisic acid, plant growth hormone
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2c
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Immunogen

S-cis, trans-abscisic acid - 4(1)- p-amino-benzoyl-hydrazone-KLH conjugate

Biochem/physiol Actions

Rat x Rat hybridoma

This cell line is the result of recloning of AFRC MAC 61. The antibody is directed against the plant growth hormone cis, trans-abscisic acid. The antibody production rate of this hybridoma has been tested at ECACC. It must be noted that this hybridoma produces less than 2μg/ml of unpurified antibody.

Preparation Note

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Maintain cultures between 3-9 x 100,000 cell/ml; 5%CO₂; 37°C. Require mouse macrophage feeder layers upon resuscitation

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.