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C8868

Cholesterol Oxidase from Brevibacterium

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

Synonym(e):

Cholesterol: oxygen oxidoreductase

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Über diesen Artikel

CAS-Nummer:
9028-76-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-842-1
MDL number:
MFCD00130783
EG-Nummer:
1.1.3.6 (BRENDA, IUBMB)
Specific activity:
≥50 units/mg protein
Recombinant:
expressed in E. coli

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Unterstützung erhalten

recombinant

expressed in E. coli

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

64 kDa

composition

Protein, ≥15% biuret

storage temp.

−20°C

Quality Level

200

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Cholesterol Oxidase from microorganisms lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

Sigma-Aldrich

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Cholesterol Oxidase from microorganisms

Cholesterol Oxidase microbial recombinant, lyophilized powder, ≥10 units/mg protein

Sigma-Aldrich

C1235

Cholesterol Oxidase microbial

Cholesterol Oxidase from microorganisms aqueous solution, ≥30 units/mg protein (biuret)

Sigma-Aldrich

C5421

Cholesterol Oxidase from microorganisms

Choline Oxidase from Arthrobacter globiformis lyophilized powder, 8-20 units/mg solid

Sigma-Aldrich

C4405

Choline Oxidase from Arthrobacter globiformis

specific activity

≥50 units/mg protein

specific activity

≥10 units/mg protein

specific activity

≥30 units/mg protein (biuret)

specific activity

8-20 units/mg solid

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

form

lyophilized powder

mol wt

64 kDa

mol wt

55 kDa

mol wt

62 kDa

mol wt

71 kDa by SDS-PAGE, 83 kDa by gel filtration

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme.

Application

Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.
Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2.[7]

Physical form

Supplied as a lyophilzed powder containing sucrose

Other Notes

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number
0000489742
0000489743
0000489743
0000489742
0000472279

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Die Dokumentenbibliothek aufrufen

Block copolymer microdomains: a novel medium for enzymatic reactions
Gupte, A., et al.
Biotechnology Progress, 7, 348-354 (2012)
Meihe Zhang et al.
Biosensors & bioelectronics, 32(1), 288-292 (2011-12-31)
A novel cholesterol biosensor was prepared based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence (ECL). Firstly, l-cysteine-reduced graphene oxide composites were modified on the surface of a glassy carbon electrode. Then, gold nanoparticles (AuNPs) were self-assembled on it. Subsequently, cholesterol oxidase
Laura Caldinelli et al.
The Journal of biological chemistry, 280(24), 22572-22581 (2005-04-09)
Cholesterol oxidase from Brevibacterium sterolicum is a monomeric flavoenzyme catalyzing the oxidation and isomerization of cholesterol to cholest-4-en-3-one. This protein is a class II cholesterol oxidases, with the FAD cofactor covalently linked to the enzyme through the His(69) residue. In
Porntip H Lolekha et al.
Clinica chimica acta; international journal of clinical chemistry, 339(1-2), 135-145 (2003-12-23)
Cholesterol oxidase is used for the determination of serum cholesterol. It can be derived from Streptomyces, Pseudomonas fluorescens, Cellulomonas, and Brevibacterium. This study compared the performance characteristics of four enzymes in the endpoint cholesterol determination. Using the Mega analyzer, we
Kwang-wook Ahn et al.
Biochemistry, 43(3), 827-836 (2004-01-21)
We investigated the dependence of cholesterol oxidase catalytic activity and membrane affinity on lipid structure in model membrane bilayers. The binding affinities of cholesterol oxidase to 100-nm unilamellar vesicles composed of mixtures of DOPC or DPPC and cholesterol are not
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Protokolle

Enzymatic Assay of Cholesterol Oxidase

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

Verwandter Inhalt

Data Sheet - C8868

Instructions

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