C8409

Anti-Cortisol antibody produced in rabbit

whole antiserum, lyophilized powder

• Synonym(e): Anti-Cortisol Antibody, Cortisol Antibody, Cortisol Antibody - Anti-Cortisol antibody produced in rabbit, Anti-Hydrocortisone
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162244
• Conjugate: unconjugated
• Clone: polyclonal
• Application: RIA
• Citations: 10

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• technique(s): radioimmunoassay: suitable (dextran coated charcoal ³H RIA)
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Beschreibung

General description

Cortisol is the principal secretary glucocorticoid of human adrenal cortex. It is produced as cortisone but converted to a biologically active form, cortisol, by the activity of enzyme 11β- hydroxysteroid dehydrogenase (11-HSD).

Immunogen

cortisol-21-thyroglobulin.

Application

Anti-Cortisol antibody has been used in Plasma hormone assay and immunofluorescence.

Anti-cortisol antibody is suitable for RIA. It has been used for ELISA to quantitate the levels of cortisol in the blood of fish. The antibody was also used for immunohistochemistry of trout gill filament sections at a working dilution of 1:5.

Biochem/physiol Actions

Cortisol is the principal secretary glucocorticoid of human adrenal cortex. It is produced as cortisone but converted to a biologically active form, cortisol, by the activity of enzyme 11β- hydroxysteroid dehydrogenase (11-HSD). Cortisol is bound to corticosteroid-binding globulin in the plasma. The organs that produce 11-HSD and involved in metabolizing and clearing it from plasma cortisol are kidney and the liver. Cortisol may be oxidised at 11-hydroxt position to result in the production of cortisone, dihydrocortisone, or tetrahydrocortisone. Cortisol and other glucocorticoids bind to the human glucocorticoid receptor (hGR) that is predominantly present in the cytoplasm. On binding, the recptor-ligand complex translocate to nucleus where the transcription of target genes is induced. Deficiency in the levels of 11-HSD are responsible for juvenile hypertension, (a feature of Apparent Mineralocorticoid Excess syndrome), edema, heart failure and hypokalemia (licorice intoxication).

Preparation Note

Reconstitute with 5 mL buffer: 0.05 M Tris-HCl, pH 8.0, 0.1 M sodium chloride, 0.1% gelatin, and 15 mM sodium azide. Store this stock at −20 °C. Working dilution (1:10) to be discarded if unused within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)