This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.
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Wichtige Dokumente
C7527
Cholinchlorid
BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
Synonym(e):
(2-Hydroxyethyl)trimethylammoniumchlorid
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100 G
€ 56,50
500 G
€ 164,00
1 KG
€ 268,00
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Größe auswählen
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100 G
€ 56,50
500 G
€ 164,00
1 KG
€ 268,00
About This Item
Lineare Formel:
(CH3)3N(Cl)CH2CH2OH
CAS-Nummer:
Molekulargewicht:
139.62
Beilstein:
3563126
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352205
PubChem Substanz-ID:
NACRES:
NA.75
Empfohlene Produkte
Biologische Quelle
synthetic (organic)
Produktlinie
BioReagent
Assay
≥98%
Form
crystals
Mol-Gew.
Mw 139.62 g/mol
Methode(n)
cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable
Farbe
white
pH-Wert
5.0-6.5 (25 °C, 140 g/L)
mp (Schmelzpunkt)
302-305 °C (dec.) (lit.)
Löslichkeit
H2O: 50 mg/mL
SMILES String
[Cl-].C[N+](C)(C)CCO
InChI
1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1
InChIKey
SGMZJAMFUVOLNK-UHFFFAOYSA-M
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Allgemeine Beschreibung
Anwendung
Cholinchlorid wird verwendet:
- als Komponente für Cholinacetyltransferase-Puffer (ChAT-Puffer) und für die Cholinaufnahme in kultivierten menschlichen Neuroblastomzellen (SK-N-SH)[3]
- zur Erstellung einer Cholin-Standardkurve zur Quantifizierung der Phospholipase D (PLD)-Aktivität[4]
- als Komponente in einer für Schnittpräparate hergestellten Lösung zur Sezierung von entorhinal-hippocampalen Hirnschnittpräparaten[5]
Substrate
Cholin-Acetyltransferase-Substrat
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Gloves
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Muscarinic acetylcholine receptors (mAChRs) are critically involved in hippocampal theta generation, but much less is known about the role of nicotinic AChRs (nAChRs). Here we provide evidence that α7 nAChRs expressed on interneurons, particularly those in oriens lacunosum moleculare (OLM)
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Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?
1 answer-
Helpful?
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Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?
1 answer-
This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w
Helpful?
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Hi, could you please provide the product information sheet for Choline chloride.
1 answer-
There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:
Certificate of Analysis
https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdfCertificate of Origin
https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921VHelpful?
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