C5992

Monoklonales Anti-pan-Zytokeratin

clone PCK-26, purified from hybridoma cell culture

• Synonym(e): Monoclonal Anti-pan-Cytokeratin, Anti-Cytokeratin, Anti-Cytokeratin - Monoclonal Anti-Cytokeratin, pan antibody produced in mouse
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: PCK-26, monoclonal
• Application: DB, ICC, IHC, WB
• Citations: 19

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PCK-26, monoclonal
• form: buffered aqueous solution
• species reactivity: human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: 10-20 μg/mL using human placenta or skin, western blot: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... Krt1(100301161) ; dog ... Krt1(444857) ; human ... KRT1(3848), KRT5(3852), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856), KRT8(3856) ; mouse ... KRT1(16678), KRT1(16678), KRT1(16678), Krt1(16678), Krt5(110308), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691), Krt8(16691) ; rat ... Krt1(300250), Krt2-5(369017), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626), Krt2-8(25626)

Beschreibung

General description

Monoclonal Anti-Pan Cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation from human epidermis. Cytokeratins are a group of at least 29 different proteins. They are characteristic of epithelial and trichocytic cells. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and is modified post translationally in terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

Immunogen

Cytokeratin aus der humanen Epidermis.

Application

Monoclonal Anti-Cytokeratin, pan antibody produced in mouse has been used in:

• immunoblotting
• immunohistochemistry
• Immunocytochemistry
• dot blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Der Antikörper erkennt ein Epitop auf Typ-II-Cytokeratinen 1, 5, 6 und 8. PCK-26 ist ein Breitspektrum-Antikörper, der spezifisch mit einer Vielfalt an normalen, reaktiven und neoplastischen Epithelgeweben reagiert. Der Antikörper reagiert mit einfachen, verhornten und unverhornten Plattenepitheln und pseudostratifizierten Epitheln.

Cytokeratin facilitates the typing of normal, metaplastic, and neoplastic cells and may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas, and neural tumors. It is also useful in detecting micrometastases in lymph nodes and other tissues, and for determining the origin of poorly differentiated tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1