C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

• Synonym(e): 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
• CAS-Nummer: 37289-07-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.5.1.24 (BRENDA, IUBMB)
• MDL number: MFCD00130789
• Specific activity: ≥100 units/mg protein

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥100 units/mg protein
• composition: Protein, ≥50% biuret
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Other Notes

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)