C-12642

Human Small Airway Epithelial Cells (HSAEpC)

500,000 cryopreserved cells

• Synonym(e): HSAEpC cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human (bronchiole)
• Morphology: (epithelial)
• Relevant disease(s): asthma

Eigenschaften

• biological source: human (bronchiole)
• packaging: pkg of 500,000 cells
• morphology: (epithelial)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): asthma
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area and are provided in a cryopreserved format. They stain positive for cytokeratin.The distal respiratory tract mainly consists of pulmonary alveoli, which are spherical outcroppings of the respiratory bronchioles and are the primary sites of gas exchange with the blood. HSAEpC are qualified for functional studies to investigate disorders such as asthma or pulmonary inflammation.

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Our HSAEpC are now also available from HLA-typed and COPD/Asthma donors


Human Small Airway Epithelial Cells (HSAEpC) serve as model cell culture systems for in vitro immune response studies. The 3D culture of HSAEpC mimics the in vivo cells effectively. The 3D cells are more viable, functional and efficient for immune response studies compared to the 2D cells.[1]

Application

Human Small Airway Epithelial Cells (HSAEpC) may be used:

• in overexpression of α_vβ₆ integrin[2]
• in cytotoxicity studies with Cannabis sativa oil extract
• in transfection studies with highly pathogenic avian influenza virus[3] and influenza A virus[4]

Biochem/physiol Actions

Bronchiole

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: > 15

Recommended Primary Cell Culture Media:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable