These Normal Human Dermal Fibroblast cells are primary cells isolated from human tissue and are not immortalized. Thawing and seeding result in passage 2, and these cells should be cryopreserved at a low passage number to maintain their proliferation potential.
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C-12300
Normal Human Dermal Fibroblasts (NHDF)
Juvenile foreskin, 500,000 cryopreserved cells
Synonym(e):
Human Dermal Fibroblast Cells, NHDF Cell Line
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Über diesen Artikel
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human foreskin (juvenile dermis)
Morphology:
(fibroblast)
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenbiological source
human foreskin (juvenile dermis)
packaging
pkg of 500,000 cells
morphology
(fibroblast)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Application
Primary Normal Human Dermal Fibroblasts (NHDF) are isolated from the dermis of juvenile foreskin and are provided in a cryopreserved format. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.
Biochem/physiol Actions
Skin
Preparation Note
Subculture Routine –Click here for more information.
Analysis Note
Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Dieser Artikel | |||
|---|---|---|---|
| description Juvenile foreskin, 500,000 cryopreserved cells | description Juvenile foreskin, 500,000 proliferating cells | description Adult, 500,000 proliferating cells | description Juvenile foreskin, 1 million cells per pellet |
| morphology (fibroblast) | morphology (fibroblast) | morphology (fibroblast) | morphology (fibroblast) |
| biological source human foreskin (juvenile dermis) | biological source human foreskin (juvenile dermis) | biological source human dermis | biological source human foreskin (juvenile dermis) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| shipped in dry ice | shipped in ambient | shipped in ambient | shipped in dry ice |
| storage temp. −196°C | storage temp. room temp | storage temp. room temp | storage temp. −20°C |
| packaging pkg of 500,000 cells | packaging pkg of 500,000 cells | packaging pkg of 500,000 cells | packaging pkg of 1,000,000 cells |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Are the Normal Human Dermal Fibroblast (C-12300) immortalized? Is it possible to expand and cryopreserve these cells? Thank you!
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