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C0888
Clostripain aus Clostridium histolyticum
≥20 units/mg solid
Synonym(e):
Clostridiopeptidase B, Proteinase
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 250 units | Warenkorb auf Verfügbarkeit prüfen | € 582,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-822-2
MDL number:
Specific activity:
≥20 units/mg solid
€ 582,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenform
lyophilized powder
Quality Level
specific activity
≥20 units/mg solid
mol wt
15.4 kDa, 41.7 kDa
impurities
salt, essentially free
storage temp.
2-8°C
Application
Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).[1]
Biochem/physiol Actions
Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.[2]
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Analysis Note
Das Enzym muss 2-3 Stunden vor Gebrauch aktiviert werden, indem es in 2.5 mM Dithiothreitol mit 1.0 mM Calciumacetat gelöst wird.
Gereinigt, weitgehend Salz-frei
Other Notes
1 U hydrolysiert 1.0 μmol BAEE pro Minute bei pH 7.6 und 25 °C in Anwesenheit von 2.5 mM DTT.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥20 units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.2-1.0 FALGPA units/mg solid | specific activity ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt 15.4 kDa, 41.7 kDa | mol wt 68-130 kDa | mol wt 68-130 kDa | mol wt 68-125 kDa |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities salt, essentially free | impurities - | impurities - | impurities - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Chang-Kyu Kim et al.
Journal of biotechnology, 131(3), 346-352 (2007-09-05)
In this study, the clostripain gene was modified and its signal sequence was replaced with that of penicillin G acylase (PGA). The core clostripain protein fused to the PGA signal peptide was also prepared. With regard to the expression of
Antibacterial activity of casein-derived peptides isolated from rabbit (Oryctolagus cuniculus) milk.
Maria Baranyi et al.
The Journal of dairy research, 70(2), 189-197 (2003-06-13)
Acid-precipitated rabbit 'whole casein' was digested by trypsin, chymotrypsin, pepsin, and clostripain to screen for possible peptides with antibacterial properties. The peptide fragments were separated by reversed-phase chromatography. The collected fractions were pooled and their antibacterial properties tested against Escherichia
Andrew K Ottens et al.
Journal of neurochemistry, 104(5), 1404-1414 (2007-11-27)
Neurotrauma, as in the case of traumatic brain injury, promotes protease over-activation characterized by the select fragmentation of brain proteins. The resulting polypeptides are indicators of biochemical processes, which can be used to study post-injury dynamics and may also be
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0888-250UN | 04061833452455 |