C0817

Monoclonal Anti-HLA-DQ antibody produced in mouse

clone HK19, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Monoclonal Anti-HLA-DQ
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: HK19, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: human
• Methode(n): flow cytometry: 5 μL using 1 × 10⁶ cells
• Isotyp: IgG2b
• UniProt-Hinterlegungsnummer: P01920
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HLA-DQB1(3119)

Beschreibung

Spezifität

Recognizes MHC Class II molecules that are preferentially expressed on the surface of B lymphocytes. The antibody reacts weakly with resting or ConA-activated T cells, monocytes, granulocytes, and erythrocytes.

Immunogen

GM-1500 human tumor cell line.

Anwendung

Monoclonal Anti-HLA-DQ antibody produced in mouse is suitable for flow cytometry at a concentration of 5 μL using 1 × 10⁶ cells.

Biochem./physiol. Wirkung

Human leukocyte antigen (HLA)-DQ is a cell surface receptor type protein found on antigen presenting cells. DQ is a αβ heterodimer of the MHC Class II type. It plays a central role in the immune system and is expressed in antigen presenting cells. HLA-DQ expression in cystic fibrosis CF is associated with inflammation and reduces response to Interferon γ (IFNγ). HLA-DQ acts as alloantibody most frequently associated with the generation of de novo donor-specific antibody (DSA) and antibody-mediated-rejection but does not support organ transplantation.

Zielbeschreibung

HLA-DQ molecules are bimolecular glycoprotein complexes of 29 kDa and 33 kDa components. The HLA-DQ antigens are present on peripheral blood lymphocytes and tonsillar and splenic mononuclear cells.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide