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C0528

Sigma-Aldrich

Casein fluorescein isothiocyanate from bovine milk

Type III, essentially salt-free, lyophilized powder

Synonym(e):

FITC-casein

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10 MG
€ 155,00
25 MG
€ 329,00
100 MG
€ 989,00

€ 155,00


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10 MG
€ 155,00
25 MG
€ 329,00
100 MG
€ 989,00

About This Item

MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.32

€ 155,00


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Typ

Type III

Form

essentially salt-free, lyophilized powder

Kennzeichnungsgrad

≥50 μg FITC per mg solid

Löslichkeit

water: 5 mg/mL, clear to hazy

Lagertemp.

2-8°C

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Anwendung

Casein fluorescein isothiocyanate from bovine milk has been used:
  • in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment[1]
  • to make a reference line in immunochromatographic devices[2]
  • in running gel for the documentation of proteinases[3]

highly sensitive protease substrate

Biochem./physiol. Wirkung

Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.[4]

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies
Kusano N, et al.
Journal of General Plant Pathology, 73(1), 66-71 (2007)
Mohamed A Seleem et al.
Journal of medicinal chemistry, 63(8), 4370-4387 (2020-04-02)
Chlamydia trachomatis is the most common sexually transmitted bacterial disease globally and the leading cause of infertility and preventable infectious blindness (trachoma) in the world. Unfortunately, there is no FDA-approved treatment specific for chlamydial infections. We recently reported two sulfonylpyridines
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food
Freese D, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 162(4), 66-72 (2012)
The supplementation of culture medium with protease improves the hatching rate of mouse embryos
Lee DR, et al.
Human Reproduction, 12(11), 2493-2498 (1997)
Emily M J Fennell et al.
Pharmacology research & perspectives, 10(4), e00993-e00993 (2022-08-06)
We recently described the identification of a new class of small-molecule activators of the mitochondrial protease ClpP. These compounds synthesized by Madera Therapeutics showed increased potency of cancer growth inhibition over the related compound ONC201. In this study, we describe

Artikel

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Questions

  1. What's the mwt of fitc-casein in kda?

    1 answer
    1. The Casein used to prepare this product is a mixture of Alpha-s1, Alpha-s2, Beta, and Kappa Casein. Combined, these forms of casein have a molecular weight range of 19 - 25.2 kDa. The Fluorescein isothiocyanate I isomer has a molecular weight of 389.38. The ratio of casein to FITC is approximately 11:1. A confirmed molecular weight has not been established.

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