B9146

5-Brom-4-Chlor-3-indolyl-β-D-Galactopyranosid

≥98%, powder, suitable for identification of lac+ bacterial colonies

• Synonym(e): 5-Brom-4-chlor-3-indolyl-β-D-galactosid, BCIG, X-Gal
• Empirische Formel (Hill-System): C₁₄H₁₅BrClNO₆
• CAS-Nummer: 7240-90-6
• Molekulargewicht: 408.63
• NACRES: NA.83
• PubChem Substance ID: 57653968
• UNSPSC Code: 12352204
• EC Number: 230-640-8
• MDL number: MFCD00005666
• Beilstein/REAXYS Number: 1402009

Eigenschaften

• Produktname: 5-Brom-4-Chlor-3-indolyl-β-D-Galactopyranosid, ≥98%
• Quality Segment: 200
• assay: ≥98%
• form: powder
• solubility: DMF: methanol (1:1): soluble 50 mg/mL
• suitability: suitable for identification of lac⁺ bacterial colonies
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• InChI key: OPIFSICVWOWJMJ-AEOCFKNESA-N

Beschreibung

Application

X-Gal ist ein chromogenes Substrat für β-Galactosidase, das einen tiefblauen Farbstoff erzeugt, der vor einem Hintergrund leicht visuell erkennbar ist. X-Gal ist das Substrat der Wahl für die Blau-Weiß-Selektion rekombinanter Bakterienkolonien mit dem lac⁺-Genotyp.

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.

Preparation Note

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)