LuceBridge ELISA Kit from rabbit serum

Name: LuceBridge ELISA Kit from rabbit serum
Brand: SIGMA

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Über diesen Artikel

NACRES:

NA.32

UNSPSC Code:

41116158

Species reactivity:

human (IgG)

Storage temp.:

2-8°C

Shipped in:

wet ice

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

antibody form

purified immunoglobulin

species reactivity

human (IgG)

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

2-8°C

Beschreibung

General description

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence of Ranibizumab. Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three dimensional structure of the protein and only a limited number of the antibodies respond. The result is a histogram which can be likened to a ‘fingerprint′ for correctly folded Ranibizumab. For a Lucentis Biosimilar, if the protein is correctly folded and glycosylated, the ‘fingerprint′ will match that of Ranibizumab. If it is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the antibody made to that exposed sequence. In this way, changes in the ‘fingerprint′ generated by the ELISA will point out differences between the Biosimilar and authentic Ranibizumab.