A7550

Achromopeptidase from Achromobacter lyticus

lyophilized powder, Protein ~5 % by biuret, 300-600 units/mg solid

• CAS-Nummer: 123175-82-6
• UNSPSC Code: 12352204
• MDL number: MFCD00130333
• EG-Nummer: 3.4.21.50 (BRENDA, IUBMB)

Eigenschaften

• form: lyophilized powder
• specific activity: 300-600 units/mg solid
• composition: Protein, ~5% biuret
• foreign activity: Collagenase, present
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀=0.6 over 2 hours at 37 °C.

Physical form

Crude powder containing salts and medium components

Other Notes

One unit will produce a change in A₆₀₀ of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves