A7080
Amphiregulin human
recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
Synonym(e):
AREG, CRDGF, SDGF, AR
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About This Item
Empfohlene Produkte
Biologische Quelle
human
Qualitätsniveau
Rekombinant
expressed in E. coli
Assay
≥97% (SDS-PAGE)
Form
lyophilized powder
Wirksamkeit
10-100 ng/mL ED50/EC50
Mol-Gew.
~11 kDa
Verpackung
pkg of 1 mg
Lagerbedingungen
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
endotoxin, tested
UniProt-Hinterlegungsnummer
Lagertemp.
−20°C
Angaben zum Gen
human ... AREG(374)
Verwandte Kategorien
Biochem./physiol. Wirkung
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
Komponenten
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
Vorsicht
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
Physikalische Form
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
Angaben zur Herstellung
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
Hinweis zur Analyse
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
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