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Merck

A4174

Sigma-Aldrich

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Rabbit Anti-Goat IgG (whole molecule)–HRP

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Konjugat

peroxidase conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

goat

Darf nicht reagieren mit

human

Methode(n)

direct ELISA: 1:10,000

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)–peroxidase antibody can be used as a secondary antibody in ovarian tissue microarray. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody reacts specifically with all goat IgG but shows no reactivity with human serum proteins.

Immunogen

Purified goat IgG.

Anwendung

Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit is suitable for use in immunoblot and immunohistochemistry. The product can also be used for direct ELISA (1:10,000).
The presence of pleiotrophin in HUVEC cell culture medium was analyzed by western blot using HRP-conjugated rabbit anti-goat IgGas the secondary at a dilution of 1:7500 in TBST.

Sonstige Hinweise

Antibody adsorbed with human serum proteins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Angaben zur Herstellung

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2


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Histone post-translational modifications (PTMs) play central roles in chromatin-templated processes. Combinations of two or more histone PTMs form unique interfaces for readout and recruitment of chromatin interacting complexes, but the genome-wide mapping of coexisting histone PTMs remains an experimentally difficult task.
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Clinical cancer research : an official journal of the American Association for Cancer Research, 11(24 Pt 1), 8585-8591 (2005-12-20)
Epithelial ovarian cancer is the most common cause of mortality from gynecologic malignancies. Due to advanced stage at diagnosis, most patients need systemic treatment in addition to surgery. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the
Evangelia Poimenidi et al.
Anticancer research, 29(1), 349-354 (2009-04-01)
Despite the fact that pleiotrophin (PTN) exhibits important biological activities related to tumor growth and angiogenesis, little is known about the regulation of its expression. In the present work, the effect of serum on PTN expression and secretion in the
Sofie Mellberg et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 23(5), 1490-1502 (2009-01-13)
To define molecular events accompanying formation of the 3-dimensional (3D) vascular tube, we have characterized gene expression during vascular endothelial growth factor (VEGF)-induced tubular morphogenesis of endothelial cells. Microarray analyses were performed comparing gene induction in growth-arrested, tube-forming endothelial cells

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