A3809

Asparaginase aus E. coli

lyophilized powder, 100-300 units/mg protein (biuret)

• Synonym(e): L-Asparagin-Amidohydrolase
• CAS-Nummer: 9015-68-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-765-3
• MDL number: MFCD00130565
• EG-Nummer: 3.5.1.1 (BRENDA, IUBMB)
• Specific activity: 100-300 units/mg protein (biuret)

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: 100-300 units/mg protein (biuret)
• composition: Protein, ≥60%
• storage temp.: 2-8°C

Beschreibung

General description

Asparaginase is a bacterial enzyme and also a chemotherapeutic drug.

Application

Asparaginase from Escherichia coli has been used:

• to compare the cytotoxic effect of L-asparaginase purified from Streptomyces brollosae NEAE-115
• as a standard in asparaginase assay to quantify asparaginase activities in various eel tissues
• to elicit amino acid deprivation

Biochem/physiol Actions

Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia . Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.

Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia . Asparaginase is allosterically regulated and is crucial for proper cell functioning .

Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle. It is capable of causing pancreatitis in leukemia patients.

Physical form

Lyophilisiertes Pulver, enthält Natriumchlorid

Preparation Note

Chromatographisch aufgereinigt

Analysis Note

Proteingehalt mittels Biuret-Reaktion bestimmt.

Other Notes

1 U setzt 1.0 μmol Ammoniak aus L-Asparagin pro Minute frei bei pH 8.6 und 37 °C.

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Warning
• Gefahrencodes: H317,H361
• Sicherheitshinweise: P201 - P280 - P302 + P352 - P308 + P313
• Hazard Classifications: Repr. 2 - Skin Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges