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A3653

Aktin aus Rindermuskel

lyophilized powder

Synonym(e):

G-Actin

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Über diesen Artikel

CAS-Nummer:
51005-14-2
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00130338
Form:
lyophilized powder
Assay:
≥90% (SDS-GE)
Biological source:
bovine muscle
Mol wt:
43 kDa

biological source

bovine muscle

Quality Segment

200

assay

≥90% (SDS-GE)

form

lyophilized powder

mol wt

43 kDa

composition

Protein, ≥80% biuret

technique(s)

western blot: suitable

UniProt accession no.

P62739

storage temp.

2-8°C

Gene Information

bovine ... LOC(528168)

General description

Actin is a highly abundant and conserved protein. It can transition between the monomeric (G-actin) and filamentous (F-actin) forms, which is influenced by external factors. Actin is expressed in smooth, skeletal and cardiac muscles.

Application

Actin from bovine muscle has been used as an internal standard and positive control during western blotting.

Biochem/physiol Actions

Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction. The protein acts as a building block of microfilaments.

Physical form

Lyophilized powder containing Tris, ATP and CaCl2

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

  1. Can you provide the protocol to polymerize the Actin from Bovine muscle?

    1 answer

    1. In the absence of salts, actin exists as a globular monomeric protein (G-actin). In the presence of ATP, a divalent cation (Ca2+ or Mg2+) and high salts (KCl, NaCl or MgCl2), the G-actin polymerizes to form a fibrous or filamentous actin (F-actin) which incorporates the cation and ATP (3,4). Dialysis of the F-actin solution (viscous) against water reverses the polymerization (2). The actin as it is supplied in product A3653 has been extensively dialyzed to be converted into G-Actin.

      This is described in the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/313/659/a3653pis.pdf

      References
      2. Sigma quality control and production.
      3. Carlier, M.-F., J. Biol. Chem., 266, 1-4 (1991) - citing Kabsch, W. et al., Nature, 347, 37-44 (1990).
      4. Merck Index, 12th Ed., #137 (1996).

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