8805209

BER human cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, IHW Number: 9093

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human blood
• Growth mode: Suspension
• Morphology: Lymphoblastoid
• Technique(s): cell culture: suitable using | mammalian

Eigenschaften

• biological source: human blood
• Quality Segment: 100
• form: liquid
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 8805209-DNA-5UG, pkg of vial of cells 8805209-1VL
• ethnicity: German
• gender: male
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• technique(s): cell culture: suitable using | mammalian

Beschreibung

General description

Consanguineous: No

Biochem/physiol Actions

A: 201
Cw: 602
B: 1302
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 101
HLA DQAP: 2.1
HLA DQA1: 201
HLA DQB1: 201
HLA DPA1: 1
HLA DPB1: 02012 0401

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

HLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 13
HLA B ief: 13.2
Bw4/bw6: 4
HLA Dw: 7
HLA DR: 7
HLA DR53: 53
HLA DQ: 2
HLA DP: 2 4

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable