The activity and protein concentration of this product varies from lot to lot and are reported in the product Certificate of Analysis. This information may then be used to determine the lot specific volume. As an example, a lot reporting a 99 mg/mL protein concentration would result in a volume of 2.53 mL for the 250 mg package size. This 250 mg package would have a total activity of 2436 units. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/76427#product-documentation
76427
Penicillin-Amidase aus E. coli
5-10 units/mg protein
Synonym(e):
Penicillin-Acylase
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
250 MG
€ 160,00
1 G
€ 470,00
€ 160,00
Warenkorb auf Verfügbarkeit prüfen
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-753-8
MDL number:
Specific activity:
5-10 units/mg protein
Biological source:
Escherichia coli
Fortfahren mit
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
Escherichia coli
form
suspension
specific activity
5-10 units/mg protein
mol wt
Mr ~70000
technique(s)
activity assay: suitable
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Quality Level
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| biological source Escherichia coli | biological source Escherichia coli | biological source Escherichia coli | biological source - |
| specific activity 5-10 units/mg protein | specific activity 50-200 units/mg protein | specific activity 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer) | specific activity 100-300 units/mg protein (biuret) |
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| form suspension | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.[1]
Application
Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N -acylhomoserine lactones) in degradation of antibiotics.[2] It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp.[3] Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.[4]
Biochem/physiol Actions
The biosynthesis of Penicillin amidase in E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by metabolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression.[5] It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.
Other Notes
1 U entspricht der Enzymmenge, die 1 μmol Benzylpenicillin pro Minute bei pH 7.6 und 37°C hydrolysiert
Characterization; In enantioselective resolution; Synthesis of ampicillin and benzylpenicillin.
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Penicillin acylase (bacterial).
T A Savidge et al.
Methods in enzymology, 43, 705-721 (1975-01-01)
V Kasche et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 365(12), 1435-1443 (1984-12-01)
Hydrophobic protein chromatography was used to prepare homogeneous fractions of penicillin amidase (EC 3.5.1.11) from E. coli. The apparent ratios of the rate constants for the deacylation of the acyl-penicillin amidase formed in the hydrolysis of phenylacetylglycine or D-phenylglycine methyl
Yi-Han Lin et al.
Molecular microbiology, 47(3), 849-860 (2003-01-22)
N-acylhomoserine lactones (AHLs) are used as signal molecules by many quorum-sensing Proteobacteria. Diverse plant and animal pathogens use AHLs to regulate infection and virulence functions. These signals are subject to biological inactivation by AHL-lactonases and AHL-acylases. Previously, little was known
A. Guy et al.
Bioorganic & Medicinal Chemistry Letters, 3, 1041-1041 (1993)
Lavinia Dunsmore et al.
Nature chemistry, 14(7), 754-765 (2022-06-29)
Natural products that contain ortho-quinones show great potential as anticancer agents but have been largely discarded from clinical development because their redox-cycling behaviour results in general systemic toxicity. Here we report conjugation of ortho-quinones to a carrier, which simultaneously masks
-
Good afternoon, I recently received Penicillin-Amidase aus E. coli 5-10 units/mg protein (250mg). Could you please guide me how much volume does it contain so that we can calculate the precise amount of concenteration available. Thank you in advance.
1 answer-
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung