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Sigma-Aldrich

Osmiumtetroxid -Lösung

suitable for electron microscopy, 2% in H2O

Synonym(e):

Osmium(IV) oxide

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About This Item

Lineare Formel:
OsO4
CAS-Nummer:
20816-12-0
Molekulargewicht:
254.23
MDL-Nummer:
MFCD00011150
UNSPSC-Code:
12171500
PubChem Substanz-ID:
57652577
NACRES:
NA.47

Form

solution

Eignung der Reaktion

reagent type: oxidant

Konzentration

2% in H2O

Methode(n)

electron microscopy: suitable

Farbe

colorless to yellow

Dichte

1.0 g/mL at 20 °C
1.0 g/mL at 20 °C

Anwendung(en)

hematology
histology

Lagertemp.

2-8°C

SMILES String

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

InChIKey

VUVGYHUDAICLFK-UHFFFAOYSA-N

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Allgemeine Beschreibung

Osmium tetroxide (OsO4) also referred to as osmium, is a heavy tetrahedral, symmetrical molecule. It is both a dye and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium tetroxide to ensure the preservation of the lipids.

Anwendung

Osmium tetroxide solution has been used as a fixative in electron microscopy.

Biochem./physiol. Wirkung

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Piktogramme

Skull and crossbonesCorrosion
GHS06,GHS05

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Lagerklassenschlüssel

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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