71196

Natriumacetat -Lösung

BioUltra, Molecular Biology, ~3 M in H₂O

• Empirische Formel (Hill-System): C₂H₃NaO₂
• CAS-Nummer: 127-09-3
• Molekulargewicht: 82.03
• NACRES: NA.21
• PubChem Substance ID: 57652229
• eCl@ss: 39021906
• UNSPSC Code: 12161700
• MDL number: MFCD00012459
• Beilstein/REAXYS Number: 3595639

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioUltra
• form: liquid
• concentration: ~3 M in H₂O
• technique(s): precipitation assay: suitable
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• pH: 7.0-8.0 (25 °C, 3 M in H₂O)
• anion traces: chloride (Cl^-): ≤10 mg/kg, phosphate (PO₄^3-): ≤5 mg/kg, sulfate (SO₄^2-): ≤20 mg/kg
• cation traces: Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤50 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
• λ: 3 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.030, λ: 280 nm A_max: ≤0.024
• SMILES string: [Na+].CC([O-])=O
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• InChI key: VMHLLURERBWHNL-UHFFFAOYSA-M

Beschreibung

Application

Sodium acetate solution has been used:

• in DNA extraction[1]
• in reverse crosslinking and DNA purification
• to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation

Biochem/physiol Actions

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients. Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.[1]

Other Notes

Basic method of DNA precipitation with ethanol.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves